PIWIL1/HIWI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06062

Novus Biologicals, part of Bio-Techne
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NBP1-06062
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PIWIL1/HIWI Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SNRKDKYDAIKKY corresponding to internal region according to NP_004755.2, NP_001177900.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PIWIL1/HIWI Antibody

Immunocytochemistry/ Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062]

Immunocytochemistry/ Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062]

Immunocytochemistry/Immunofluorescence: PIWIL1/HIWI Antibody [NBP1-06062] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-06062]

Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-06062]

Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-06062] - Staining of paraffin embedded Human Testis. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: PIWIL1/HIWI Antibody [NBP1-06062]

Flow Cytometry: PIWIL1/HIWI Antibody [NBP1-06062]

Flow Cytometry: PIWIL1/HIWI Antibody [NBP1-06062] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for PIWIL1/HIWI Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:64000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIWIL1/HIWI

The PIWIL1 gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organ

Long Name

Piwi-like Protein 1

Alternate Names

HIWI, MIWI

Entrez Gene IDs

9271 (Human); 57749 (Mouse); 363912 (Rat)

Gene Symbol

PIWIL1

UniProt

NP_004755.2 (Human)

Additional PIWIL1/HIWI Products

Product Documents for PIWIL1/HIWI Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIWIL1/HIWI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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