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PIWIL1/HIWI Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55299

Novus Biologicals, part of Bio-Techne
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NBP1-55299

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to PIWIL1(piwi-like 1 (Drosophila)) The peptide sequence was selected from the N terminal of PIWIL1. Peptide sequence FQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFR. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PIWIL1/HIWI Antibody

Western Blot: PIWIL1/HIWI Antibody [NBP1-55299]

Western Blot: PIWIL1/HIWI Antibody [NBP1-55299]

Western Blot: PIWIL1/HIWI Antibody [NBP1-55299] - Antibody Titration: 1 ug/ml Human heart.
Immunohistochemistry: PIWIL1/HIWI Antibody [NBP1-55299]

Immunohistochemistry: PIWIL1/HIWI Antibody [NBP1-55299]

Immunohistochemistry: PIWIL1/HIWI Antibody [NBP1-55299] - Human Adult heart Observed Staining: Cytoplasmic Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.
Western Blot: PIWIL1/HIWI Antibody [NBP1-55299]

Western Blot: PIWIL1/HIWI Antibody [NBP1-55299]

Western Blot: PIWIL1/HIWI Antibody [NBP1-55299] - Human Stomach, concentration 0.2-1 ug/ml.

Applications for PIWIL1/HIWI Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIWIL1/HIWI

PIWIL1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PI

Long Name

Piwi-like Protein 1

Alternate Names

HIWI, MIWI

Gene Symbol

PIWIL1

UniProt

Additional PIWIL1/HIWI Products

Product Documents for PIWIL1/HIWI Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIWIL1/HIWI Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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