PJA2/Praja2 Antibody (1G11)
Novus Biologicals, part of Bio-Techne | Catalog # H00009867-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PJA2 (NP_055634, 302 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. REKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGA
Reactivity Notes
Human. Other species not tested.
Localization
Cytoplasmic. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Localizes at the cytoplasmic side of endoplasmi
Specificity
PJA2 - praja 2, RING-H2 motif containing
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for PJA2/Praja2 Antibody (1G11)
![Western Blot: PJA2/Praja2 Antibody (1G11) [H00009867-M01]](https://resources.bio-techne.com/images/products/PJA2-Praja2-Antibody-1G11-Western-Blot-H00009867-M01-img0002.jpg "Western Blot: PJA2/Praja2 Antibody (1G11) [H00009867-M01]")
Western Blot: PJA2/Praja2 Antibody (1G11) [H00009867-M01]
Western Blot: PJA2/Praja2 Antibody (1G11) [H00009867-M01] - Analysis of PJA2 expression in transfected 293T cell line by PJA2 monoclonal antibody (M01), clone 1G11.Lane 1: PJA2 transfected lysate (Predicted MW: 78.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PJA2/Praja2 Antibody (1G11) [H00009867-M01] - Detection limit for recombinant GST tagged PJA2 is 0.1 ng/ml as a capture antibody.
Applications for PJA2/Praja2 Antibody (1G11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PJA2/Praja2
Alternate Names
E3 ubiquitin-protein ligase Praja-2, EC 6.3.2, EC 6.3.2.-, KIAA0438praja2, Neurodap1, praja ring finger 2, Praja2, RING finger protein 131praja 2, RING-H2 motif containing, RNF131
Entrez Gene IDs
9867 (Human)
Gene Symbol
PJA2
UniProt
Additional PJA2/Praja2 Products
Product Documents for PJA2/Praja2 Antibody (1G11)
Product Specific Notices for PJA2/Praja2 Antibody (1G11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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