PKA C alpha Antibody (1D7)

Novus Biologicals, part of Bio-Techne | Catalog # H00005566-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKA C alpha Antibodies »

Product Specifications

Immunogen

PRKACA (AAH39846, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMV

Specificity

PRKACA - protein kinase, cAMP-dependent, catalytic, alpha (1D7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKA C alpha Antibody (1D7)

Proximity Ligation Assay: PKA C alpha Antibody (1D7) [H00005566-M02]

Proximity Ligation Assay: PKA C alpha Antibody (1D7) [H00005566-M02]

Proximity Ligation Assay: PKA C alpha Antibody (1D7) [H00005566-M02] - Analysis of protein-protein interactions between MAP3K3 and PRKACA. HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-PRKACA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: PKA C alpha Antibody (1D7) [H00005566-M02] - Detection limit for recombinant GST tagged PRKACA is approximately 0.1ng/ml as a capture antibody.

Applications for PKA C alpha Antibody (1D7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKA C alpha

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Alternate Names

Cs, Pkaca, PKCA1, PKCD, PRKACA

Entrez Gene IDs

5566 (Human)

Gene Symbol

PRKACA

UniProt

AAH39846 (Human)

Additional PKA C alpha Products

Product Documents for PKA C alpha Antibody (1D7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA C alpha Antibody (1D7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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