PKA C alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57042

Novus Biologicals, part of Bio-Techne
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NBP2-57042
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKA C alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: KQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKA C alpha Antibody

Western Blot: PKA C alpha Antibody [NBP2-57042]

Western Blot: PKA C alpha Antibody [NBP2-57042]

Western Blot: PKA C alpha Antibody [NBP2-57042] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: PKA C alpha Antibody [NBP2-57042]

Immunocytochemistry/ Immunofluorescence: PKA C alpha Antibody [NBP2-57042]

Immunocytochemistry/Immunofluorescence: PKA C alpha Antibody [NBP2-57042] - Staining of human cell line MCF7 shows localization to cytosol & cytokinetic bridge. Antibody staining is shown in green.

Applications for PKA C alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA C alpha

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha

Alternate Names

Cs, Pkaca, PKCA1, PKCD, PRKACA

Gene Symbol

PRKACA

Additional PKA C alpha Products

Product Documents for PKA C alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKA C alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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