PKA R2 Antibody (OTI1F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02520

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F8

Concentration

0.74 mg/ml

View all PKA R2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA R2 Antibody (OTI1F8)

Western Blot: PKA R2 Antibody (OTI1F8) [NBP2-02520]

Western Blot: PKA R2 Antibody (OTI1F8) [NBP2-02520]

Western Blot: PKA R2 Antibody (1F8) [NBP2-02520] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA R2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKA R2.
Immunocytochemistry/Immunofluorescence: PKA R2 Antibody (1F8) [NBP2-02520] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA R2.
Immunohistochemistry-Paraffin: PKA R2 Antibody (OTI1F8) [NBP2-02520]

Immunohistochemistry-Paraffin: PKA R2 Antibody (OTI1F8) [NBP2-02520]

Immunohistochemistry-Paraffin: PKA R2 Antibody (1F8) [NBP2-02520] - Staining of paraffin-embedded Human prostate tissue using anti-PKA R2 mouse monoclonal antibody.
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Applications for PKA R2 Antibody (OTI1F8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.74 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA R2

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq].

Alternate Names

protein kinase, cAMP-dependent, regulatory, type II, alpha, RII-alpha subunit

Entrez Gene IDs

5576 (Human)

Gene Symbol

PRKAR2A

Additional PKA R2 Products

Product Documents for PKA R2 Antibody (OTI1F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA R2 Antibody (OTI1F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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