PKA R2 [p Ser99] Antibody (SC54-04)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67571
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SC54-04
Concentration
1 mg/ml
Product Summary for PKA R2 [p Ser99] Antibody (SC54-04)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser99 of Human PKA R2 aa 82-126 / 404. (SwissProt: P13861 Human; SwissProt: P12367 Mouse; SwissProt: P12368 Rat)
Localization
Cell membrane, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PKA R2 [p Ser99] Antibody (SC54-04)
Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Applications for PKA R2 [p Ser99] Antibody (SC54-04)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKA R2
Alternate Names
protein kinase, cAMP-dependent, regulatory, type II, alpha, RII-alpha subunit
Gene Symbol
PRKAR2A
Additional PKA R2 Products
Product Documents for PKA R2 [p Ser99] Antibody (SC54-04)
Product Specific Notices for PKA R2 [p Ser99] Antibody (SC54-04)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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