PKA RI beta Antibody (1F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005575-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Sandwich ELISA Capture, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKA RI beta Antibodies »

Product Specifications

Immunogen

PRKAR1B (NP_002726, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPV

Specificity

PRKAR1B (1F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKA RI beta Antibody (1F8)

Western Blot: PKA RI beta Antibody (1F8) [H00005575-M05]

Western Blot: PKA RI beta Antibody (1F8) [H00005575-M05]

Western Blot: PKA RI beta Antibody (1F8) [H00005575-M05] - Analysis of PRKAR1B expression in HepG2 (Cat # L019V1).
Immunohistochemistry-Paraffin: PKA RI beta Antibody (1F8) [H00005575-M05]

Immunohistochemistry-Paraffin: PKA RI beta Antibody (1F8) [H00005575-M05]

Immunohistochemistry-Paraffin: PKA RI beta Antibody (1F8) [H00005575-M05] - Analysis of monoclonal antibody to PRKAR1B on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml
Sandwich ELISA Capture: PKA RI beta Antibody (1F8) [H00005575-M05]

Sandwich ELISA Capture: PKA RI beta Antibody (1F8) [H00005575-M05]

Sandwich ELISA Capture: PKA RI beta Antibody (1F8) [H00005575-M05] - Detection limit for recombinant GST tagged PKA RI beta is 0.03 ng/ml as a capture antibody.

Applications for PKA RI beta Antibody (1F8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKA RI beta

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.

Long Name

cAMP-dependent Protein Kinase Regulatory Type I beta

Alternate Names

PRKAR1B, RIbeta

Entrez Gene IDs

5575 (Human)

Gene Symbol

PRKAR1B

OMIM

176911 (Human)

UniProt

NP_002726 (Human)

Additional PKA RI beta Products

Product Documents for PKA RI beta Antibody (1F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKA RI beta Antibody (1F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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