PKA RI beta Antibody (OTI2A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00634

Novus Biologicals, part of Bio-Techne
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NBP2-00634
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A3

Concentration

0.57 mg/ml

View all PKA RI beta Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA RI beta Antibody (OTI2A3)

Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634]

Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634]

Western Blot: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA regulatory subunit I beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKA regulatory subunit I beta.
Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634]

Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634]

Immunocytochemistry/Immunofluorescence: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA regulatory subunit I beta.
Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]

Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634]

Immunohistochemistry-Paraffin: PKA RI beta Antibody (OTI2A3) [NBP2-00634] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKA regulatory subunit I beta mouse monoclonal antibody.
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Applications for PKA RI beta Antibody (OTI2A3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.57 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA RI beta

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM].

Long Name

cAMP-dependent Protein Kinase Regulatory Type I beta

Alternate Names

PRKAR1B, RIbeta

Entrez Gene IDs

5575 (Human)

Gene Symbol

PRKAR1B

Additional PKA RI beta Products

Product Documents for PKA RI beta Antibody (OTI2A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA RI beta Antibody (OTI2A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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