PKA RI beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87451

Novus Biologicals, part of Bio-Techne
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NBP1-87451
NBP1-87451-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKA RI beta Antibodies »

Product Summary for PKA RI beta Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKA RI beta Antibody

Western Blot: PKA RI beta Antibody [NBP1-87451]

Western Blot: PKA RI beta Antibody [NBP1-87451]

Western Blot: PKA RI beta Antibody [NBP1-87451] - Analysis in control (vector only transfected HEK293T lysate) and PRKAR1B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451]

Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451]

Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for PKA RI beta Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA RI beta

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.

Long Name

cAMP-dependent Protein Kinase Regulatory Type I beta

Alternate Names

PRKAR1B, RIbeta

Gene Symbol

PRKAR1B

Additional PKA RI beta Products

Product Documents for PKA RI beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA RI beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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