PKC delta Antibody (2E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00005580-M06

Novus Biologicals, part of Bio-Techne
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H00005580-M06
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC delta Antibodies »

Product Specifications

Immunogen

PRKCD (NP_006245, 577 a.a. ~ 676 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

Localization

Cytoplasmic

Specificity

PRKCD - protein kinase C, delta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKC delta Antibody (2E12)

Western Blot: PKC delta Antibody (2E12) [H00005580-M06]

Western Blot: PKC delta Antibody (2E12) [H00005580-M06]

Western Blot: PKC delta Antibody (2E12) [H00005580-M06] - Western Blot analysis of PRKCD expression in A-431 ( Cat # L015V1 ).
Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (2E12) [H00005580-M06]

Immunocytochemistry/ Immunofluorescence: PKC delta Antibody (2E12) [H00005580-M06]

Immunocytochemistry/Immunofluorescence: PKC delta Antibody (2E12) [H00005580-M06] - Analysis of monoclonal antibody to PRKCD on A-431 cell. Antibody concentration 10 ug/ml
ELISA: PKC delta Antibody (2E12) [H00005580-M06]

ELISA: PKC delta Antibody (2E12) [H00005580-M06]

ELISA: PKC delta Antibody (2E12) [H00005580-M06] - Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.

Applications for PKC delta Antibody (2E12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKC delta

Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Long Name

Protein Kinase C delta

Alternate Names

PRKCD

Entrez Gene IDs

5580 (Human)

Gene Symbol

PRKCD

OMIM

176977 (Human)

UniProt

NP_006245 (Human)

Additional PKC delta Products

Product Documents for PKC delta Antibody (2E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC delta Antibody (2E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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