PKC delta [p Ser299] Antibody (S06-4A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19827

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19827-100ul
NBP3-19827-50ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-4A5

Concentration

0.3 mg/ml

View all PKC delta Antibodies »

Product Specifications

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser299 of human PKC delta (Uniprot # Q05655)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC delta [p Ser299] Antibody (S06-4A5)

Western Blot: PKC delta [p Ser299] Antibody (S06-4A5) [NBP3-19827] - Western blot detection of phospho-PKC delta (Ser299) in A549, HL-60 cell lysates using NBP3-19827 (1:1000 diluted). Predicted band size: 78kDa. Observed band size: 78kDa.
Immunohistochemistry-Paraffin: PKC delta [p Ser299] Antibody (S06-4A5) [NBP3-19827] - Immunohistochemistry of PKC delta (phospho-Ser299) in paraffin-embedded Human tonsil using NBP3-19827 at dilution 1/50

Applications for PKC delta [p Ser299] Antibody (S06-4A5)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC delta

PKC-delta has a biological activity which is closely related to Pkc1, and PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-delta desensitizes the pathway by regulating an aspect of G protein function (1). PKC-delta phosphorylates hRad9 in vitro and in cells exposed to genotoxic agents. It has been shown that PKC-delta is required for binding of hRad9 to Bcl-2. In concert with these results, inhibition of PKC-delta attenuates Rad9-mediated apoptosis. These findings demonstrate that PKC-delta is responsible for the regulation of Rad9 in the Hus1-Rad1 complex and in the apoptotic response to DNA damage (2). It has been reported a mechanism for the regulation of peripheral B-cell survival by serine/threonine protein kinase Cdelta (PKC-delta): spontaneous death of resting B cells is regulated by nuclear localization of PKC-delta that contributes to phosphorylation of histone H2B at serine 14 (S14-H2B) (3).

Long Name

Protein Kinase C delta

Alternate Names

PRKCD

Gene Symbol

PRKCD

Additional PKC delta Products

Product Documents for PKC delta [p Ser299] Antibody (S06-4A5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC delta [p Ser299] Antibody (S06-4A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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