PKC epsilon Antibody (OTI4G8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73438

Novus Biologicals, part of Bio-Techne
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Size / Price
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NBP2-73438
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Conjugate
Catalog #
Unconjugated
NBP2-03620
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Alexa Fluor 350
NBP2-73438AF350
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Alexa Fluor 405
NBP2-73438AF405
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Alexa Fluor 488
NBP2-73438AF488
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Alexa Fluor 532
NBP2-73438AF532
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Alexa Fluor 594
NBP2-73438AF594
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Alexa Fluor 647
NBP2-73438AF647
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Alexa Fluor 700
NBP2-73438AF700
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Alexa Fluor 750
NBP2-73438AF750
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Allophycocyanin
NBP2-73438APC
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Allophycocyanin/Cy7
NBP2-73438APCCY7
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Biotin
NBP2-73438B
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CoraFluor 1
NBP2-73438CL1
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DyLight 350
NBP2-73438UV
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DyLight 405
NBP2-73438V
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DyLight 488
NBP2-73438G
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DyLight 550
NBP2-73438R
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DyLight 594
NBP2-73438DL594
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DyLight 650
NBP2-73438C
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DyLight 680
NBP2-73438FR
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DyLight 755
NBP2-73438IR
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FITC
NBP2-73438F
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HRP
NBP2-73438H
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Janelia Fluor 525
NBP2-73438JF525
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Janelia Fluor 549
NBP2-73438JF549
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Janelia Fluor 585
NBP2-73438JF585
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Janelia Fluor 635
NBP2-73438JF635
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Janelia Fluor 646
NBP2-73438JF646
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Janelia Fluor 669
NBP2-73438JF669
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mFluor Violet 450 SE
NBP2-73438MFV450
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mFluor Violet 500 SE
NBP2-73438MFV500
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mFluor Violet 610 SE
NBP2-73438MFV610
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PE
NBP2-73438PE
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PE/Atto594
NBP2-73438PEATT594
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PE/Cy5.5
NBP2-73438PECY55
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PE/Cy7
NBP2-73438PECY7
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PerCP
NBP2-73438PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4G8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PKC epsilon Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRKCE (NP_005391) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

83.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC epsilon Antibody (OTI4G8) - Azide and BSA Free

Western Blot: PKC epsilon Antibody (OTI4G8)Azide and BSA Free [NBP2-73438]

Western Blot: PKC epsilon Antibody (OTI4G8)Azide and BSA Free [NBP2-73438]

Western Blot: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKC epsilon (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKC epsilo
Immunocytochemistry/ Immunofluorescence: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438]

Immunocytochemistry/ Immunofluorescence: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438]

Immunocytochemistry/Immunofluorescence: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKC epsilon.
Immunohistochemistry: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438]

Immunohistochemistry: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438]

Immunohistochemistry: PKC epsilon Antibody (OTI4G8) - Azide and BSA Free [NBP2-73438] - Staining of paraffin-embedded Human lymphoma tissue using anti-PKC epsilon mouse monoclonal antibody.
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Applications for PKC epsilon Antibody (OTI4G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC epsilon

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Long Name

Protein Kinase C epsilon

Alternate Names

PKCE, PRKCE

Gene Symbol

PRKCE

Additional PKC epsilon Products

Product Documents for PKC epsilon Antibody (OTI4G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC epsilon Antibody (OTI4G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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