PKC gamma Antibody (5F2X3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16611

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16611-100ul
NBP3-16611-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5F2X3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC gamma Antibodies »

Product Summary for PKC gamma Antibody (5F2X3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 604-697 of human PKC gamma (P05129). GEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC gamma Antibody (5F2X3)

Western Blot: PKC gamma Antibody (5F2X3) [NBP3-16611]

Western Blot: PKC gamma Antibody (5F2X3) [NBP3-16611]

Western Blot: PKC gamma Antibody (5F2X3) [NBP3-16611] - Western blot analysis of extracts of various cell lines, using PKC gamma Rabbit mAb (NBP3-16611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for PKC gamma Antibody (5F2X3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC gamma

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Long Name

Protein Kinase C gamma

Alternate Names

PKCC, PKCG, PRKCG, SCA14

Gene Symbol

PRKCG

Additional PKC gamma Products

Product Documents for PKC gamma Antibody (5F2X3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC gamma Antibody (5F2X3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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