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PKC gamma Antibody (HL2226) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25606

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25606

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2226

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PKC gamma Antibody (HL2226) - Azide and BSA Free

Immunogen

Recombinant fragmemt of human PKC gamma.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC gamma Antibody (HL2226) - Azide and BSA Free

PKC gamma Antibody (HL2226) - Azide and BSA Free

Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] -

Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PKC gamma Antibody (HL2226) - Azide and BSA Free

Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] -

Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - PKC proteins (10 ng) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PKC gamma Antibody (HL2226) - Azide and BSA Free

Immunohistochemistry-Paraffin: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] -

Immunohistochemistry-Paraffin: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - PKC gamma antibody [HL2226] detects PKC gamma protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PKC gamma stained by PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for PKC gamma Antibody (HL2226) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC gamma

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Long Name

Protein Kinase C gamma

Alternate Names

PKCC, PKCG, PRKCG, SCA14

Gene Symbol

PRKCG

Additional PKC gamma Products

Product Documents for PKC gamma Antibody (HL2226) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKC gamma Antibody (HL2226) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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