PKC mu Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13292

Novus Biologicals, part of Bio-Techne
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NBP3-13292
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRKD1/nPKC mu Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse PKC mu. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PKC mu Antibody

Western Blot: PKC mu Antibody [NBP3-13292]

Western Blot: PKC mu Antibody [NBP3-13292]

Western Blot: PKC mu Antibody [NBP3-13292] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membranes were blotted with PKC mu antibody (NBP3-13292) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PKC mu Antibody [NBP3-13292]

Immunocytochemistry/ Immunofluorescence: PKC mu Antibody [NBP3-13292]

Immunocytochemistry/Immunofluorescence: PKC mu Antibody [NBP3-13292] - PKC mu antibody detects PKC mu protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PKC mu protein stained by PKC mu antibody (NBP3-13292) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Applications for PKC mu Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRKD1/nPKC mu

PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).

Long Name

Protein Kinase D1

Alternate Names

PKCM, PRKD1

Gene Symbol

PRKD1

Additional PRKD1/nPKC mu Products

Product Documents for PKC mu Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKC mu Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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