PKC theta [p Thr538] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05454

Novus Biologicals, part of Bio-Techne
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NBP3-05454
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC theta Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around T538 of human PRKCQ (NP_006248.1). TNTFC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC theta [p Thr538] Antibody - Azide and BSA Free

Western Blot: PKC theta [p Thr538] AntibodyAzide and BSA Free [NBP3-05454]

Western Blot: PKC theta [p Thr538] AntibodyAzide and BSA Free [NBP3-05454]

Western Blot: PKC theta [p Thr538] Antibody [NBP3-05454] - Western blot analysis of extracts of Jurkat and HeLa cells, using PKC theta antibody (NBP3-05454) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. Hela cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for PKC theta [p Thr538] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKC theta

Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, episilon , zeta, eta and theta). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and episilon are independent of Ca++. On the other hand, nPKC and episilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Gene Symbol

PRKCQ

Additional PKC theta Products

Product Documents for PKC theta [p Thr538] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC theta [p Thr538] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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