PKC zeta [p Thr560] Antibody (1B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26399

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC zeta Antibodies »

Product Summary for PKC zeta [p Thr560] Antibody (1B10)

Immunogen

A synthesized peptide derived from Human Phospho-PKC zeta [p Thr560] [UniProt Q05513]

Modification

p Thr 560

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC zeta [p Thr560] Antibody (1B10)

PKC zeta [p Thr560] Antibody (1B10)

Western Blot: PKC zeta [p Thr560] Antibody (1B10) [NBP3-26399] -

Western Blot: PKC zeta [p Thr560] Antibody (1B10) [NBP3-26399] - Positive Western Blot detected in A549 whole cell lysate.
All lanes: PKC zeta [p Thr560] Antibody at 1.55ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 68 KDa
Observed band size: 68 KDa

Applications for PKC zeta [p Thr560] Antibody (1B10)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PKC zeta

Protein kinase C (PKC), a class of serine/threonine kinases activated by Ca2+ and/or phospholipids, is involved in a variety of cellular processes such as proliferation, differentiation and secretion. PKC zeta exhibits a constitutive kinase activity which is independent of Ca2+, phosphatidylserine and diacylglycerol. Arachidonic acid alone or a combination of gamma-linolenic acid and phosphatidylserine slightly enhance PKC zeta activity. PKC zeta, in contrast to PKC alpha, is insensitive to PKC inhibitors known to interfere either with the regulatory or the catalytic domain and cannot be activated by phorbol ester treatment of NIH 3T3 cells or insect cells, overexpressing the respective PKC isoenzyme (1). Results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway. This phosphorylation and activation of PKC zeta by PDK-1 is enhanced in the presence of Ptdins-3,4-5-P3. PKCs are enzymes that are regulated at the plasma membrane, and a membrane-targeted PKC zeta is constitutively active in the absence of agonist stimulation. The association between PKC zeta and PDK-1 reveals extensive cross-talk between enzymes in the PI 3-kinase signaling pathway. (2)

Long Name

Protein Kinase C zeta

Alternate Names

PKC2, PRKCZ

Gene Symbol

PRKCZ

Additional PKC zeta Products

Product Documents for PKC zeta [p Thr560] Antibody (1B10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC zeta [p Thr560] Antibody (1B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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