PKC zeta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82147

Novus Biologicals, part of Bio-Techne
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NB100-82147
NB100-82147-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PKC zeta Antibodies »

Product Specifications

Immunogen

Peptide sequence around amino acids 408~412 ( T-S-T-F-C) derived from PKC zeta

Specificity

Detects endogenous levels of total PKC zeta protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC zeta Antibody

Western Blot: PKC zeta Antibody [NB100-82147]

Western Blot: PKC zeta Antibody [NB100-82147]

Western Blot: PKC zeta Antibody [NB100-82147] - Analysis of extract from 293 cells and Hela using PKC zeta antibody.
Immunohistochemistry-Paraffin: PKC zeta Antibody [NB100-82147]

Immunohistochemistry-Paraffin: PKC zeta Antibody [NB100-82147]

Immunohistochemistry-Paraffin: PKC zeta Antibody [NB100-82147] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKC zeta antibody.

Applications for PKC zeta Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27064903).

Reviewed Applications

Read 1 review rated 4 using NB100-82147 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC zeta

Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involved. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu.

Long Name

Protein Kinase C zeta

Alternate Names

PKC2, PRKCZ

Entrez Gene IDs

5590 (Human); 18762 (Mouse); 25522 (Rat)

Gene Symbol

PRKCZ

UniProt

Q05513

Additional PKC zeta Products

Product Documents for PKC zeta Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC zeta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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