PKLR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32314

Novus Biologicals, part of Bio-Techne
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NBP1-32314
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (91%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKLR Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKLR. The exact sequence is proprietary.

Reactivity Notes

Dog (89%).

Specificity

Pyruvate kinase from liver and RBC.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKLR Antibody

Western Blot: PKLR Antibody [NBP1-32314]

Western Blot: PKLR Antibody [NBP1-32314]

Western Blot: PKLR Antibody [NBP1-32314] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:500.
Western Blot: PKLR Antibody [NBP1-32314]

Western Blot: PKLR Antibody [NBP1-32314]

Western Blot: PKLR Antibody [NBP1-32314] - 30 ug Raw264. 7 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: PKLR Antibody [NBP1-32314]

Western Blot: PKLR Antibody [NBP1-32314]

Western Blot: PKLR Antibody [NBP1-32314] - A. 30 ug K562 whole cell lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.
View 1 more images

Applications for PKLR Antibody

Application
Recommended Usage

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKLR

Pyruvate kinase is also known as ATP:pyruvate phosphotransferase. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney. PK1 is found also in liver. During fetal development, PK3 changes to PK1 in the liver. PK1 is a tetramer composed of two dissimilar polypeptides of somewhat different molecular weight. It is an allosteric enzyme exhibiting cooperative binding for phosphoenolpyruvate and sensitivity to fructose-1,6-diphosphate. PK3 also is a tetrameric protein but, unlike PK1, all subunits are alike and, not unexpectedly, there is no cooperative behavior. The enzyme is insensitive to fructose-1,6-diphosphate. Patients with deficiency of red cell PK have normal PK2 and PK3. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. Ab38237 recognizes the M1/M2 isoform of pyruvate kinase

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Gene Symbol

PKLR

Additional PKLR Products

Product Documents for PKLR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKLR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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