PKM2 Antibody (1B10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42686
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B10
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human PKM2 (1-531aa) purified from E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for PKM2 Antibody (1B10) - BSA Free
Western Blot: PKM2 Antibody (1B10)BSA Free [NBP2-42686]
Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (1B10) - BSA Free [NBP2-42686]
Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Immunocytochemistry/ Immunofluorescence: PKM2 Antibody (1B10) - BSA Free [NBP2-42686]
Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Applications for PKM2 Antibody (1B10) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKM2
Long Name
Pyruvate Kinase, Muscle
Alternate Names
CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK
Gene Symbol
PKM
UniProt
Additional PKM2 Products
Product Documents for PKM2 Antibody (1B10) - BSA Free
Product Specific Notices for PKM2 Antibody (1B10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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