PKM2 Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-20589
Key Product Details
Species Reactivity
Rabbit
Applications
ELISA, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
PKM2 [Rabbit Muscle] (Uniprot: P11974)
Reactivity Notes
Cross reactivity against PKM2 from other tissues and species may occur but have not been specifically determined
Localization
Cytoplasmic
Specificity
This product has been reported to react with all forms of pyruvate kinase (pan M-PK).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]
Scientific Data Images for PKM2 Antibody [Biotin]
Western Blot: PKM2 Antibody [Biotin] [NB120-20589] - Western Blot of PKM2 antibody [Biotin].Lane 1: Opal Prestain Molecular Weight Marker.Lane 2: 50ng of PKM2.Primary Antibody: Goat Anti-PKM2 Biotin at 1ug/mL o/n @ 4C.Secondary Antibody: Streptavidin-HRP: S000-03 at 1:40,000 for 30 min at RT.Blocking: 30 min at RT.
Applications for PKM2 Antibody [Biotin]
Application
Recommended Usage
ELISA
1:1000-1:5000
Western Blot
1:200-1:1000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: PKM2
Long Name
Pyruvate Kinase, Muscle
Alternate Names
CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK
Gene Symbol
PKM
UniProt
Additional PKM2 Products
Product Documents for PKM2 Antibody [Biotin]
Product Specific Notices for PKM2 Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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