PKM2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48308

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Bovine

Applications

Validated:

Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PKM2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450).

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25416385).

Localization

Cytoplasm. Nucleus. Note: Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKM2 Antibody - BSA Free

Western Blot: PKM2 AntibodyBSA Free [NBP1-48308]

Western Blot: PKM2 AntibodyBSA Free [NBP1-48308]

Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression profile of PKM isoforms in tissues from mouse organs. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S2b. Results are presented as the mean +/- SD (** P < 0.01). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep08647) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308]

Immunocytochemistry/ Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308]

Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.
Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308]

Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308]

Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Staining of PKM2 in mouse liver tissue.
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Applications for PKM2 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

5 ug/ml

Immunoblotting

reported in scientific literature (PMID 25416385)

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

reported in scientific literature (PMID 21620138)

Simple Western

1:12.5

Western Blot

0.5 ug/ml
Application Notes
In Western blot, a band is seen at approx. 60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 3 reviews rated 5 using NBP1-48308 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKM2

PKM2 (pyruvate kinase isozymes M1/M2), an isoform of pyruvate kinase, is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP and is expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Other three pyruvate kinases includes PKL (liver and kidney), PKR (erythrocytes) and PKM1, (adult muscle, brain, bladder, and fibroblasts) which exist as stable tetramers, whereas, PKM2 subunits form tetramers as well as dimers, the dimeric form with higher Km value for PEP substrate stays inactive at physiological concentrations of PEP. The ratio between highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. Predominately localized in the cytoplasm, PKM2 translocate to nucleus upon apoptotic stimuli and this translocation mediated cell death is caspase independent, isoform-specific and independent of its enzymatic activity.

Long Name

Pyruvate Kinase, Muscle

Alternate Names

CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK

Entrez Gene IDs

5315 (Human); 18746 (Mouse)

Gene Symbol

PKM

UniProt

P14618 (Human), P52480 (Mouse)

Additional PKM2 Products

Product Documents for PKM2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKM2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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