PKM2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19852
![Western Blot: PKM2 Antibody [NBP2-19852]](https://resources.bio-techne.com/images/products/PKM2-Antibody-Western-Blot-NBP2-19852-img0009.jpg "Western Blot: PKM2 Antibody [NBP2-19852]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PKM2. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PKM2 Antibody
![Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852]](https://resources.bio-techne.com/images/products/PKM2-Antibody-Immunohistochemistry-Paraffin-NBP2-19852-img0006.jpg "Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852]")
Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852]
Immunohistochemistry-Paraffin: PKM2 Antibody [NBP2-19852] - antibody detects PKM2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Pyruvate Kinase (muscle) antibody dilution: 1:500.![Western Blot: PKM2 Antibody [NBP2-19852]](https://resources.bio-techne.com/images/products/PKM2-Antibody-Western-Blot-NBP2-19852-img0008.jpg "Western Blot: PKM2 Antibody [NBP2-19852]")
Western Blot: PKM2 Antibody [NBP2-19852]
Western Blot: PKM2 Antibody [NBP2-19852] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.
Applications for PKM2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PKM2
Long Name
Pyruvate Kinase, Muscle
Alternate Names
CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK
Gene Symbol
PKM
UniProt
Additional PKM2 Products
Product Documents for PKM2 Antibody
Product Specific Notices for PKM2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.