PKN1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98761
![Immunocytochemistry/ Immunofluorescence: PKN1 Antibody [NBP2-98761]](https://resources.bio-techne.com/images/products/PKN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98761-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PKN1 Antibody [NBP2-98761]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PKN1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PKN1 Antibody
Immunocytochemistry/ Immunofluorescence: PKN1 Antibody [NBP2-98761]
Immunocytochemistry/Immunofluorescence: PKN1 Antibody [NBP2-98761] - Immunofluorescence staining of PKN1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-HUMAN PKN1 polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Western Blot: PKN1 Antibody [NBP2-98761] -
Western Blot: PKN1 Antibody [NBP2-98761] - Lane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:125 kDaApplications for PKN1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKN1
Long Name
Serine/Threonine Protein Kinase N1
Alternate Names
DBK, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1
Gene Symbol
PKN1
Additional PKN1 Products
Product Documents for PKN1 Antibody
Product Specific Notices for PKN1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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