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PKNOX2 Antibody (4B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00063876-M01

Novus Biologicals, part of Bio-Techne
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H00063876-M01

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PKNOX2 (NP_071345, 116 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PELDNLMVKAIQVLRIHLLELEKVNELCKDFCNRYITCFKTKMHSDNLLRNDLGGPYSPNQPSINLHSQDLLQNSPNSMSGVSNNPQGIVVPASALQQGN

Specificity

PKNOX2 - PBX/knotted 1 homeobox 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for PKNOX2 Antibody (4B6)

Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01]

Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01]

Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - PKNOX2 monoclonal antibody (M01), clone 4B6. Analysis of PKNOX2 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: PKNOX2 Antibody (4B6) [H00063876-M01]

Immunocytochemistry/ Immunofluorescence: PKNOX2 Antibody (4B6) [H00063876-M01]

Immunocytochemistry/Immunofluorescence: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of monoclonal antibody to PKNOX2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01]

Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01]

Western Blot: PKNOX2 Antibody (4B6) [H00063876-M01] - Analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi ( Cat # H00063876-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody (M01), clone 4B6 (Cat # H00063876-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for PKNOX2 Antibody (4B6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validaton and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKNOX2

Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain andplay fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acidloop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2within the homeodomain (Imoto et al., 2001 (PubMed 11549286)).(supplied by OMIM)

Alternate Names

homeobox protein PKNOX2, PBX/knFLJ13074, PBX/knPREP2Homeobox protein PREP-2

Entrez Gene IDs

63876 (Human)

Gene Symbol

PKNOX2

Additional PKNOX2 Products

Product Documents for PKNOX2 Antibody (4B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKNOX2 Antibody (4B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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