PLA2G6 Antibody

Western Blot: PLA2G6 Antibody [NBP1-81586]

Western Blot: PLA2G6 Antibody [NBP1-81586] - Effects of APPWT and APPOSK overexpression on the levels of the major phospholipase A2s in the brains of old mice. Cerebrum samples from non-Tg, APPWT and APPOSK were lyophilized, pulverized, and homogenized in NP40 buffer using a cooled bead beater. Total protein concentrations from NP40 supernatants were estimated by BCA protein assay. Representative Western blots using multiple antibodies against PLA2. Image collected and cropped by CiteAb from the following publication (https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-017-0460-6), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: PLA2G6 Antibody [NBP1-81586]

Immunocytochemistry/Immunofluorescence: PLA2G6 Antibody [NBP1-81586] - Staining of human cell line A-431 shows localization to microtubule organizing center. Antibody staining is shown in green.

Immunohistochemistry: PLA2G6 Antibody [NBP1-81586]

Immunohistochemistry: PLA2G6 Antibody [NBP1-81586] - Non-Tg mouse brains were dissected, fixed in 4% paraformaldehyde, cryoprotected, frozen, & sectioned coronally (8 um sections). Representative immunofluorescence images taken from brain coronal sections (Bregma -2) using phospho-cPLA2 (yellow) antibody and DAPI (blue) (a) or and iPLA2 (yellow) and NeuN (red) antibodies (b). Images show a whole hemibrain section using a 20x objective on a Nikon A1R VAAS inverted confocal (left panels). NeuN and iPLA2 co-localize within the perinucleus of pyramidal neurons (PN, white arrows), but not within the perinucleus of interneurons (IN). iPLA2 staining is also observed within pyramidal dendritic arborization (right panels) Image collected and cropped by CiteAb from the following publication (https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-017-0460-6), licensed under a CC-BY license.