Plakophilin 1 Antibody (10B2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54526
![Western Blot: Plakophilin 1 Antibody (10B2)Azide and BSA Free [NBP2-54526]](https://resources.bio-techne.com/images/products/Plakophilin-1-Antibody-10B2-Azide-and-BSA-Free-Western-Blot-NBP2-54526-img0001.jpg "Western Blot: Plakophilin 1 Antibody (10B2)Azide and BSA Free [NBP2-54526]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 10B2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
N-terminus of human Plakophilin 1 protein (Uniprot: Q13835)
Epitope
aa 1-27
Localization
Cell Surface and nuclear
Specificity
Recognizes a protein of 75kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53326).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Plakophilin 1 Antibody (10B2) - Azide and BSA Free
Western Blot: Plakophilin 1 Antibody (10B2)Azide and BSA Free [NBP2-54526]
Western Blot: Plakophilin 1 Antibody (10B2) - Azide and BSA Free [NBP2-54526] - Western Blot of U87, HeLa and A431 cell lysates using Plakophilin 1 Antibody (10B2)Applications for Plakophilin 1 Antibody (10B2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Plakophilin 1
Alternate Names
B6PBand 6 protein, MGC138829, plakophilin 1 (ectodermal dysplasia/skin fragility syndrome), plakophilin-1
Gene Symbol
PKP1
Additional Plakophilin 1 Products
Product Documents for Plakophilin 1 Antibody (10B2) - Azide and BSA Free
Product Specific Notices for Plakophilin 1 Antibody (10B2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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