Skip to main content

Plakophilin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72054

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-72054

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RGTAQYSSQKSVEER corresponding to internal region according to NP_004563.2.

Reactivity Notes

Expected from sequence similarity: Rat, Canine, Bovine

Specificity

This antibody is expected to recognize both reported isoforms (NP_001005242.2; NP_004563.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Plakophilin 2 Antibody

Western Blot: Plakophilin 2 Antibody [NBP1-72054]

Western Blot: Plakophilin 2 Antibody [NBP1-72054]

Western Blot: Plakophilin 2 Antibody [NBP1-72054] - (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-72054]

Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-72054]

Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-72054] - 3.75ug/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Plakophilin 2 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-1ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3-1.0 ug/ml
Application Notes
Western blot: Approx 100kDa band observed in Mouse Heart lysates (calculated MW of 88.1kDa according to Mouse NP_080439.1 and 87.8kDa according to Rat NP_001093969.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Prostate.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Plakophilin 2

Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.

Alternate Names

ARVD9, MGC177501, plakophilin 2, plakophilin-2

Entrez Gene IDs

5318 (Human); 67451 (Mouse)

Gene Symbol

PKP2

Additional Plakophilin 2 Products

Product Documents for Plakophilin 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Plakophilin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...