Plasminogen Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-930
![Western Blot: Plasminogen Antibody [NB600-930]](https://resources.bio-techne.com/images/products/Plasminogen-Antibody-Western-Blot-NB600-930-img0006.jpg "Western Blot: Plasminogen Antibody [NB600-930]")
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Applications
Validated:
ELISA, Western Blot
Cited:
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Plasminogen [Human Plasma] (Uniprot: P00747)
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 15075185).
Localization
Secreted
Specificity
Cross reactivity against Plasminogen from other sources is unknown.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Plasminogen Antibody
Western Blot: Plasminogen Antibody [NB600-930]
Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.![Western Blot: Plasminogen Antibody [NB600-930]](https://resources.bio-techne.com/images/products/Plasminogen-Antibody-Western-Blot-NB600-930-img0005.jpg "Western Blot: Plasminogen Antibody [NB600-930]")
Western Blot: Plasminogen Antibody [NB600-930]
Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
Plasminogen Antibody
Goat anti Plasminogen antibody ( lot 6571) was used to detect Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in ). Detection shown was using Dylight 649 conjugated Donkey anti goat ( lot 20834 1:10K in TBS/) 1 hr RT. Images were collected using the BioRad VersaDoc System.Applications for Plasminogen Antibody
Application
Recommended Usage
ELISA
1:20000-1:100000
Western Blot
1:2000-1:10000
Application Notes
This product has been tested by western blot. This product is suitable to be assayed against 1.0 ug of Plasminogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reviewed Applications
Read 2 reviews rated 5 using NB600-930 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Plasminogen
Additional Plasminogen Products
Product Documents for Plasminogen Antibody
Product Specific Notices for Plasminogen Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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