Plasminogen Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19859

Novus Biologicals, part of Bio-Techne
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NBP2-19859
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Plasminogen Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Plasminogen. The exact sequence is proprietary.

Reactivity Notes

Bovine (81%), Dog (81%), Pig (80%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Plasminogen Antibody

Western Blot: Plasminogen Antibody [NBP2-19859]

Western Blot: Plasminogen Antibody [NBP2-19859]

Western Blot: Plasminogen Antibody [NBP2-19859] - Rat tissue extract (50ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Plasminogen Antibody [NBP2-19859]

Immunocytochemistry/ Immunofluorescence: Plasminogen Antibody [NBP2-19859]

Immunocytochemistry/Immunofluorescence: Plasminogen Antibody [NBP2-19859] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry: Plasminogen Antibody [NBP2-19859]

Immunohistochemistry: Plasminogen Antibody [NBP2-19859]

Immunohistochemistry: Plasminogen Antibody [NBP2-19859] - Expression of plasminogen/plasmin (NBP2-19859) in normal and PCOS mouse ovaries. Representative histological images are from normal (control) (Ai, Aiii) or PCOS ovaries (Aii, Aiv) stained with an anti-plasminogen/plasmin antibody. The scale bar represents 500 um for Ai, Aii and 100 um for Aiii, Aiv. Right: Top: Negative control for plasminogen/plasmin on mouse liver; Middle: Graphical representation comparing the percentage of ovarian staining (Area Fraction ROI) for plasminogen/plasmin and plasminogen alone between normal and PCOS ovaries for 6 replicates of each experimental group (mean +/- SD) and Bottom: Negative control for plasminogen alone on mouse liver Image collected and cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/s12958-019-0472-0) licensed under a CC-BY license.
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Applications for Plasminogen Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Plasminogen

Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU; MIM 191840) and tissue plasminogen activator (PLAT; MIM 173370). The main function of plasmin is to dissolve fibrin (see, e.g., FGA, MIM 134820) clots. Plasmin, like trypsin, belongs to the family of serine proteinases (Miyata et al., 1982 [PubMed 6216475]; Forsgren et al., 1987 [PubMed 3030813]).[supplied by OMIM]

Alternate Names

Plg

Gene Symbol

PLG

UniProt

P00747

Additional Plasminogen Products

Product Documents for Plasminogen Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Plasminogen Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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