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PLC-gamma 1 Antibody (2F3D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61790

Novus Biologicals, part of Bio-Techne
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NBP2-61790
NBP2-61790-0.025ml

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2F3D11

Format

BSA Free

Concentration

1 mg/ml

Product Summary for PLC-gamma 1 Antibody (2F3D11) - BSA Free

Immunogen

Purified recombinant fragment of human PLC-gamma 1 (AA: 39-181) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

89.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLC-gamma 1 Antibody (2F3D11) - BSA Free

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 39-181)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against human PLCG1 (AA: 39-181) recombinant protein. (Expected MW is 43 kDa)
Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11)BSA Free [NBP2-61790]

Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mouse mAb against Jurkat (1), K562 (2), A431 (3), Hela (4), and PC-12 (5) cell lysate.

Applications for PLC-gamma 1 Antibody (2F3D11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 1

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.;

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Gene Symbol

PLCG1

Additional PLC-gamma 1 Products

Product Documents for PLC-gamma 1 Antibody (2F3D11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLC-gamma 1 Antibody (2F3D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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