PLC-gamma 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33036

Novus Biologicals, part of Bio-Techne
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NBP1-33036
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLC-gamma 2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PLC-gamma 2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLC-gamma 2 Antibody

Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody [NBP1-33036]

Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody [NBP1-33036]

Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody [NBP1-33036] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-33036]

Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-33036]

Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-33036] - Paraffin-embedded Gastric carcinoma, using antibody at 1:500 dilution.
PLC-gamma 2 Antibody

Western Blot: PLC-gamma 2 Antibody [NBP1-33036] -

Western Blot: PLC-gamma 2 Antibody [NBP1-33036] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLCG2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for PLC-gamma 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLC-gamma 2

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgammaII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgammaII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgammaII (2). It has also been shown that PLCgammaII was required for receptor activator of NF-kappaB ligand-induced (RANKL-induced) osteoclastogenesis by differentially regulating nuclear factor of activated T cells c1 (NFATc1), activator protein-1 (AP1), and NF-kappaB (3).

Long Name

Phospholipase C gamma 2

Alternate Names

PLCG2, PLCgamma 2

Gene Symbol

PLCG2

Additional PLC-gamma 2 Products

Product Documents for PLC-gamma 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PLC-gamma 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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