PLC-gamma 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87558

Novus Biologicals, part of Bio-Techne
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NBP1-87558
NBP1-87558-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLC-gamma 2 Antibodies »

Product Summary for PLC-gamma 2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TKENNMKYWEKNQSIAIELSDLVVYCKPTSKTKDNLENPDFREIRSFVETKADSIIRQKPVDLLKYNQKGLTRVYPKGQRVDSSNYDPF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLC-gamma 2 Antibody

Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody [NBP1-87558]

Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody [NBP1-87558]

Immunocytochemistry/Immunofluorescence: PLC-gamma 2 Antibody [NBP1-87558] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-87558]

Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-87558]

Immunohistochemistry-Paraffin: PLC-gamma 2 Antibody [NBP1-87558] - Staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.
PLC-gamma 2 Antibody Western Blot: PLC-gamma 2 Antibody Antibody [NBP1-87558]

Western Blot: PLC-gamma 2 Antibody Antibody [NBP1-87558]

Analysis using NBP1-87558 (A) shows similar pattern to independent antibody NBP1-86030 (B).

Applications for PLC-gamma 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 2

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgammaII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgammaII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgammaII (2). It has also been shown that PLCgammaII was required for receptor activator of NF-kappaB ligand-induced (RANKL-induced) osteoclastogenesis by differentially regulating nuclear factor of activated T cells c1 (NFATc1), activator protein-1 (AP1), and NF-kappaB (3).

Long Name

Phospholipase C gamma 2

Alternate Names

PLCG2, PLCgamma 2

Gene Symbol

PLCG2

Additional PLC-gamma 2 Products

Product Documents for PLC-gamma 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLC-gamma 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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