PLC-gamma 2 [p Tyr1217] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13270

Novus Biologicals, part of Bio-Techne
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NBP3-13270
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLC-gamma 2 Antibodies »

Product Summary for PLC-gamma 2 [p Tyr1217] Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1217 of human PLC-gamma 2. The exact sequence is proprietary.

Modification

p Tyr 1217

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PLC-gamma 2 [p Tyr1217] Antibody

Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270]

Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270]

Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr1217) antibody (NBP3-13270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270]

Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270]

Western Blot: PLC-gamma 2 [p Tyr1217] Antibody [NBP3-13270] - Untreated (-) and treated (+) Raji whole cell extract (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLC-gamma 2 (phospho Tyr1217) antibody (NBP3-13270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for PLC-gamma 2 [p Tyr1217] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 2

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgammaII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgammaII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgammaII (2). It has also been shown that PLCgammaII was required for receptor activator of NF-kappaB ligand-induced (RANKL-induced) osteoclastogenesis by differentially regulating nuclear factor of activated T cells c1 (NFATc1), activator protein-1 (AP1), and NF-kappaB (3).

Long Name

Phospholipase C gamma 2

Alternate Names

PLCG2, PLCgamma 2

Gene Symbol

PLCG2

Additional PLC-gamma 2 Products

Product Documents for PLC-gamma 2 [p Tyr1217] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PLC-gamma 2 [p Tyr1217] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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