Pleckstrin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1343

Novus Biologicals, part of Bio-Techne
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NB100-1343
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Pleckstrin Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EWIKAIQMASRTGK corresponding to C-Terminus according to NP_002655.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pleckstrin Antibody

Western Blot: Pleckstrin Antibody [NB100-1343]

Western Blot: Pleckstrin Antibody [NB100-1343]

Western Blot: Pleckstrin Antibody [NB100-1343] - Staining of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Pleckstrin Antibody [NB100-1343]

Immunocytochemistry/ Immunofluorescence: Pleckstrin Antibody [NB100-1343]

Immunocytochemistry/Immunofluorescence: Pleckstrin Antibody [NB100-1343] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Pleckstrin Antibody [NB100-1343]

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NB100-1343]

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NB100-1343] - Staining of paraffin embedded Human Spleen. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Applications for Pleckstrin Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

3.75 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.01 - 0.3 ug/mL
Application Notes
WB: Approx. 45 kDa band observed in human peripheral blood mononucleocyte lysates (calculated MW of 40.1 kDa band according to NP_002655.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pleckstrin

Pleckstrin is the major protein kinase C substrate of platelets however its exact function is not known.

Alternate Names

FLJ27168, p47, P47Platelet 47 kDa protein, pleckstrin

Entrez Gene IDs

5341 (Human)

Gene Symbol

PLEK

UniProt

P08567 (Human)

Additional Pleckstrin Products

Product Documents for Pleckstrin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pleckstrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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