Pleckstrin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87370

Novus Biologicals, part of Bio-Techne
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NBP1-87370
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pleckstrin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGH

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pleckstrin Antibody

Western Blot: Pleckstrin Antibody [NBP1-87370]

Western Blot: Pleckstrin Antibody [NBP1-87370]

Western Blot: Pleckstrin Antibody [NBP1-87370] - Analysis in control (vector only transfected HEK293T lysate) and PLEK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370]

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370]

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370] - Staining in human bone marrow and pancreas tissues using anti-PLEK antibody. Corresponding PLEK RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370]

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370]

Immunohistochemistry-Paraffin: Pleckstrin Antibody [NBP1-87370] - Staining of human bone marrow shows high expression.
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Applications for Pleckstrin Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pleckstrin

Pleckstrin is the major protein kinase C substrate of platelets however its exact function is not known.

Alternate Names

FLJ27168, p47, P47Platelet 47 kDa protein, pleckstrin

Gene Symbol

PLEK

Additional Pleckstrin Products

Product Documents for Pleckstrin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pleckstrin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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