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Pleiotrophin/PTN Antibody (5C3)

Novus Biologicals, part of Bio-Techne | Catalog # H00005764-M01

Novus Biologicals, part of Bio-Techne
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H00005764-M01

Key Product Details

Species Reactivity

Validated:

Human, Rat, Chicken

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5C3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PTN (NP_002816, 45 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDCGEWQWSVCVPTSGDCGLGTREGTRTGAECKQTMKTQRCKIPCNWKKQFGAECKYQFQAWGECDLNTALKTRTGSLKRALHNAECQKTVTISKPCGKLTKPKPQAESK

Specificity

PTN - pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Pleiotrophin/PTN Antibody (5C3)

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01]

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01]

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01]

Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01]

Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of monoclonal antibody to PTN on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.
Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01]

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01]

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - PTN monoclonal antibody (M01), clone 5C3 Analysis of PTN expression in Hela S3 NE.

Applications for Pleiotrophin/PTN Antibody (5C3)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pleiotrophin/PTN

Heparin binding mitogenic protein. Has neurite extension activity

Alternate Names

HARP, HBNF, HNGF-8, OSF-1, PTN

Entrez Gene IDs

5764 (Human)

Gene Symbol

PTN

OMIM

162095 (Human)

Additional Pleiotrophin/PTN Products

Product Documents for Pleiotrophin/PTN Antibody (5C3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pleiotrophin/PTN Antibody (5C3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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