Pleiotrophin/PTN Antibody (JE47-40)

Western Blot: Pleiotrophin/PTN Antibody (JE47-40) [NBP3-32877]

Western Blot: Pleiotrophin/PTN Antibody (JE47-40) [NBP3-32877] - Western blot analysis of Pleiotrophin/PTN on different lysates with Rabbit anti-Pleiotrophin/PTN antibody (NBP3-32877) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 3 minutes 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32877) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Pleiotrophin/PTN Antibody (JE47-40) [NBP3-32877]

Immunohistochemistry: Pleiotrophin/PTN Antibody (JE47-40) [NBP3-32877] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Pleiotrophin/PTN antibody (NBP3-32877) at 1/3,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32877) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.