PLEKHA3 Antibody (OTI5D11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00768
![Flow Cytometry: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]](https://resources.bio-techne.com/images/products/PLEKHA3-Antibody-5D11-Flow-Cytometry-NBP2-00768-img0003.jpg "Flow Cytometry: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]")
![Western Blot: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]](https://resources.bio-techne.com/images/products/PLEKHA3-Antibody-5D11-Western-Blot-NBP2-00768-img0004.jpg "Western Blot: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5D11
Concentration
1.1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PLEKHA3 (NP_061964) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PLEKHA3 Antibody (OTI5D11)
Western Blot: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]
Western Blot: PLEKHA3 Antibody (5D11) [NBP2-00768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLEKHA3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEKHA3.![Immunocytochemistry/ Immunofluorescence: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]](https://resources.bio-techne.com/images/products/PLEKHA3-Antibody-5D11-Immunocytochemistry-Immunofluorescence-NBP2-00768-img0005.jpg "Immunocytochemistry/ Immunofluorescence: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]")
Immunocytochemistry/ Immunofluorescence: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]
Immunocytochemistry/Immunofluorescence: PLEKHA3 Antibody (5D11) [NBP2-00768] - Immunofluorescent staining of HeLa cells using anti-PLEKHA3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLEKHA3 Antibody (5D11) [NBP2-00768] Staining of paraffin-embedded Human lymph node tissue using anti-PLEKHA3 mouse monoclonal antibody.
![Immunohistochemistry-Paraffin: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]](https://resources.bio-techne.com/images/products/PLEKHA3-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-00768-img0006.jpg "Immunohistochemistry-Paraffin: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]")
![Flow Cytometry: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]](https://resources.bio-techne.com/images/products/PLEKHA3-Antibody-5D11-Flow-Cytometry-NBP2-00768-img0001.jpg "Flow Cytometry: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]")
![Flow Cytometry: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]](https://resources.bio-techne.com/images/products/PLEKHA3-Antibody-5D11-Flow-Cytometry-NBP2-00768-img0002.jpg "Flow Cytometry: PLEKHA3 Antibody (OTI5D11) [NBP2-00768]")
Applications for PLEKHA3 Antibody (OTI5D11)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PLEKHA3
Alternate Names
FAPP-1, FAPP1PH domain-containing family A member 3, FLJ20067, four-phosphate-adaptor protein 1, Phosphatidylinositol-four-phosphate adapter protein 1, Phosphoinositol 4-phosphate adapter protein 1, pleckstrin homology domain containing, family A (phosphoinositide bindingspecific) member 3, pleckstrin homology domain-containing family A member 3, pleckstrin homology domain-containing, family A (phosphoinositide bindingspecific) member 3
Entrez Gene IDs
65977 (Human)
Gene Symbol
PLEKHA3
Additional PLEKHA3 Products
Product Documents for PLEKHA3 Antibody (OTI5D11)
Product Specific Notices for PLEKHA3 Antibody (OTI5D11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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