Plexin D1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12915

Novus Biologicals, part of Bio-Techne
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NBP3-12915
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Plexin D1 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Plexin D1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Plexin D1 Antibody

Western Blot: Plexin D1 Antibody [NBP3-12915]

Western Blot: Plexin D1 Antibody [NBP3-12915]

Western Blot: Plexin D1 Antibody [NBP3-12915] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Plexin D1 antibody (NBP3-12915) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP3-12915]

Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP3-12915]

Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP3-12915] - Plexin D1 antibody detects Plexin D1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebral cortex. Green: Plexin D1 stained by Plexin D1 antibody (NBP3-12915) diluted at 1:250. Blue: Fluoroshield with DAPI.

Applications for Plexin D1 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Plexin D1

Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors. Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins. The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and approximately 84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron. Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus. Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct. Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities. Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development. In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal. Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E. Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands.

Alternate Names

PLXND1

Gene Symbol

PLXND1

Additional Plexin D1 Products

Product Documents for Plexin D1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Plexin D1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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