PLGF Antibody (3B10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-96418
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3B10
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for PLGF Antibody (3B10) - Azide and BSA Free
Immunogen
Recombinant human PlGF-2
Specificity
P1GF-2 (3B10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PLGF Antibody (3B10) - Azide and BSA Free
Western Blot: PLGF Antibody (3B10)Azide and BSA Free [NB110-96418]
Western Blot: PLGF Antibody (3B10) [NB110-96418] - Figure 1: Western analysis with recombinant human PlGF-1 and PlGF-2 derived from insect cells. The monoclonal mouse anti-human PlGF antibody #3B10 recognizes solely the PlGF-2 isoform.Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]
Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - Immunofluorescence staining of mouse VEGF-A and PLGF in primary mouse endothelial cells (mEC) using a monoclonal antibodies directed against human VEGF-A and human PLGF [NB110-96418], respectively. As secondary antibody goat anti-mouse ALEXA Flour 488 (1:600) was used.Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]
Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of PlGF in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and human dermal normal fibroblasts with monoclonal mouse anti-human PlGF #3B10. The cells were pretreated with 0,1% Triton X100. Control with goat anti-mouse ALEXA Flour 488.Applications for PLGF Antibody (3B10) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1-2 ug/ml
Western Blot
1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PlGF
Long Name
Placenta Growth Factor
Alternate Names
PGF, PGFL
Gene Symbol
PGF
Additional PlGF Products
Product Documents for PLGF Antibody (3B10) - Azide and BSA Free
Product Specific Notices for PLGF Antibody (3B10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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