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PLGF Antibody (3B10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96418

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for PLGF Antibody (3B10) - Azide and BSA Free

Immunogen

Recombinant human PlGF-2

Specificity

P1GF-2 (3B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PLGF Antibody (3B10) - Azide and BSA Free

Western Blot: PLGF Antibody (3B10)Azide and BSA Free [NB110-96418]

Western Blot: PLGF Antibody (3B10)Azide and BSA Free [NB110-96418]

Western Blot: PLGF Antibody (3B10) [NB110-96418] - Figure 1: Western analysis with recombinant human PlGF-1 and PlGF-2 derived from insect cells. The monoclonal mouse anti-human PlGF antibody #3B10 recognizes solely the PlGF-2 isoform.
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]

Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - Immunofluorescence staining of mouse VEGF-A and PLGF in primary mouse endothelial cells (mEC) using a monoclonal antibodies directed against human VEGF-A and human PLGF [NB110-96418], respectively. As secondary antibody goat anti-mouse ALEXA Flour 488 (1:600) was used.
Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]

Immunocytochemistry/ Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418]

Immunocytochemistry/Immunofluorescence: PLGF Antibody (3B10) - Azide and BSA Free [NB110-96418] - PLGF Antibody (3B10) [NB110-96418] - Immunofluorescence staining of PlGF in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and human dermal normal fibroblasts with monoclonal mouse anti-human PlGF #3B10. The cells were pretreated with 0,1% Triton X100. Control with goat anti-mouse ALEXA Flour 488.

Applications for PLGF Antibody (3B10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1-2 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PlGF

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It belongs to the cysteine-knot superfamily. PLGF is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Gene Symbol

PGF

Additional PlGF Products

Product Documents for PLGF Antibody (3B10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLGF Antibody (3B10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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