PLOD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38770
![Western Blot: PLOD1 Antibody [NBP2-38770]](https://resources.bio-techne.com/images/products/PLOD1-Antibody-Western-Blot-NBP2-38770-img0006.jpg "Western Blot: PLOD1 Antibody [NBP2-38770]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: FVSLFFQRLLRLHYPQKHMRLFIHNHEQHHKAQVEEFLAQHGSEYQSVKLVGPEVRMANADARN
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27119146). Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PLOD1 Antibody
![Western Blot: PLOD1 Antibody [NBP2-38770]](https://resources.bio-techne.com/images/products/PLOD1-Antibody-Western-Blot-NBP2-38770-img0007.jpg "Western Blot: PLOD1 Antibody [NBP2-38770]")
Western Blot: PLOD1 Antibody [NBP2-38770]
Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.![Western Blot: PLOD1 Antibody [NBP2-38770]](https://resources.bio-techne.com/images/products/PLOD1-Antibody-Western-Blot-NBP2-38770-img0005.jpg "Western Blot: PLOD1 Antibody [NBP2-38770]")
Western Blot: PLOD1 Antibody [NBP2-38770]
Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY license.Applications for PLOD1 Antibody
Application
Recommended Usage
Western Blot
0.04-0.4 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:34400365).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PLOD1
Alternate Names
2-oxoglutarate 5-dioxygenase 1, EC 1.14.11.4, Ehlers-Danlos syndrome type VI), FLJ42041, LH1, LLH, lysine hydroxylase, Lysyl hydroxylase 1, PLODLH, procollagen-lysine, procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1, procollagen-lysine, 2-oxoglutarate 5-dioxygenase
Gene Symbol
PLOD1
UniProt
Additional PLOD1 Products
Product Documents for PLOD1 Antibody
Product Specific Notices for PLOD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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