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PLRG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48650

Novus Biologicals, part of Bio-Techne
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NBP2-48650
NBP2-48650-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GPGVALTADTKIQRMPSESAAQSLAVALPLQTKADANRTAPSGSEYRHPGASDRPQPTAMNSIVMETGNTKNSALMAKKAPTMPKPQWH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLRG1 Antibody

Western Blot: PLRG1 Antibody [NBP2-48650]

Western Blot: PLRG1 Antibody [NBP2-48650]

Western Blot: PLRG1 Antibody [NBP2-48650] - Analysis using Anti-PLRG1 antibody NBP2-48650 (A) shows similar pattern to independent antibody NBP1-87225 (B).
Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP2-48650]

Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP2-48650]

Immunocytochemistry/Immunofluorescence: PLRG1 Antibody [NBP2-48650] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: PLRG1 Antibody [NBP2-48650]

Immunohistochemistry-Paraffin: PLRG1 Antibody [NBP2-48650]

Immunohistochemistry-Paraffin: PLRG1 Antibody [NBP2-48650] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.

Applications for PLRG1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLRG1

Pleiotropic regulator 1 (PLRG1) has been shown to be a part of sub-spliceosomal protein complexes. It is highly conserved across species and is essential for pre-mRNA splicing. PLRG1 interacts with human CDC5. This interaction has also been shown to be essential to pre-mRNA splicing. PLRG1 is also known as PRL1, and MGC110980.

Alternate Names

MGC110980, pleiotropic regulator 1 (PRL1 homolog, Arabidopsis), PRL1Arabidopsis homolog)

Gene Symbol

PLRG1

Additional PLRG1 Products

Product Documents for PLRG1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLRG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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