PMCA1 Antibody (3E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00000490-M01

Novus Biologicals, part of Bio-Techne
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H00000490-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PMCA1 Antibodies »

Product Specifications

Immunogen

ATP2B1 (NP_001673, 1 a.a. ~ 97 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKT

Specificity

ATP2B1 - ATPase, Ca++ transporting, plasma membrane 1. May react with other PMCA ATPases.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PMCA1 Antibody (3E2)

Sandwich ELISA: PMCA1 Antibody (3E2) [H00000490-M01] - Detection limit for recombinant GST tagged ATP2B1 is approximately 0.3ng/ml as a capture antibody.

Applications for PMCA1 Antibody (3E2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PMCA1

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

ATPase, Ca++ transporting, plasma membrane 1, EC 3.6.3, EC 3.6.3.8, plasma membrane calcium ATPase, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium pump, Plasma membrane calcium pump isoform 1, plasma membrane calcium-ATPase, PMCA1kb, PMCA1plasma membrane calcium-transporting ATPase 1

Entrez Gene IDs

490 (Human)

Gene Symbol

ATP2B1

OMIM

108731 (Human)

UniProt

NP_001673 (Human)

Additional PMCA1 Products

Product Documents for PMCA1 Antibody (3E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PMCA1 Antibody (3E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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