PMCA1 Antibody (JE34-05)

Western Blot: PMCA1 Antibody (JE34-05) [NBP3-32830]

Western Blot: PMCA1 Antibody (JE34-05) [NBP3-32830] - Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/1,000 dilution.

Lane 1: A549 cell lysate (no heat)
Lane 2: A549 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 135 kDa
Observed band size: 135-250 kDa

Exposure time: 20 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PMCA1 Antibody (JE34-05) [NBP3-32830]

Immunohistochemistry: PMCA1 Antibody (JE34-05) [NBP3-32830] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32830) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.