PMCA4 Antibody (2G8)

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07]

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. siRNA mediated inhibition of PMCA4b expression downregulates RP-220 mediated MAPK signaling; left panel: RP-220 mediated ERK and p38 activation in HK-2 cells transfected with non-targeting siRNA, p = phosphorylated, representative immunoblot; middle panel: Inhibition of RP-220 mediated ERK and p38 activation in HK-2 cells transfected with PMCA4b siRNA, representative blot; right panel: quantification of phosphorylated ERK (p-ERK), signals normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control; n = 3, * = P<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07]

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Lack of effect of siRNA mediated inhibition of PMCA4b expression on epidermal growth factor (EGF)- mediated MAPK signaling; left panel: EGF (100 ng/ml) mediated ERK, p38 activation and c-Jun N-Terminal Kinase (JNK) in HK-2 cells transfected with non-targeting siRNA, p = phosphorylated, representative blot; right panel: EGF-mediated ERK, p38 and JNK activation in HK-2 cells unaffected by transfection with PMCA4b siRNA and downregulation of PMCA4b expression; representative blot (n = 3). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.

Sandwich ELISA: PMCA4 Antibody (2G8) [H00000493-M07] - Detection limit for recombinant GST tagged ATP2B4 is 1 ng/ml as a capture antibody.

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] -

Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE & visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b & renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b & renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.