PMEL17/SILV Antibody (JE42-47)

Western Blot: PMEL17/SILV Antibody (JE42-47) [NBP3-32586]

Western Blot: PMEL17/SILV Antibody (JE42-47) [NBP3-32586] - Western blot analysis of PMEL17/SILV on SK-MEL-28 cell lysates with Rabbit anti-PMEL17/SILV antibody (NBP3-32586) at 1/1,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 70 kDa
Observed band size: 100 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32586) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PMEL17/SILV Antibody (JE42-47) [NBP3-32586]

Immunohistochemistry: PMEL17/SILV Antibody (JE42-47) [NBP3-32586] - Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-PMEL17/SILV antibody (NBP3-32586) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32586) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.