Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PMEL/783

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Recombinant human PMEL17/SILV protein (Uniprot: P40967)

Localization

Cytoplasmic

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341] - Formalin-fixed, paraffin-embedded human Testis stained with gp100 Mouse Monoclonal Antibody (PMEL/783).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted [NBP2-48341] - Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/783).

Applications for PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PMEL17/SILV Antibody (PMEL/783) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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