PMEL17/SILV Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-41098
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CPIGENSPLLSGQQ corresponding to C-Terminus according to NP_008859.1.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 24572365).
Marker
Melanosome Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PMEL17/SILV Antibody
![Western Blot: PMEL17/SILV Antibody [NB100-41098]](https://resources.bio-techne.com/images/products/PMEL17-SILV-Antibody-Western-Blot-NB100-41098-img0001.jpg "Western Blot: PMEL17/SILV Antibody [NB100-41098]")
Western Blot: PMEL17/SILV Antibody [NB100-41098]
Western Blot: PMEL17/SILV Antibody [NB100-41098] - Staining of Mouse Skin lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody [NB100-41098]](https://resources.bio-techne.com/images/products/PMEL17-SILV-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41098-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody [NB100-41098]")
Immunocytochemistry/ Immunofluorescence: PMEL17/SILV Antibody [NB100-41098]
Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody [NB100-41098] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for PMEL17/SILV Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Peptide ELISA
Detection limit 1:16000
Western Blot
1 - 3 ug/mL
Application Notes
WB: Approx. 26 kDa band observed in human skin lysates, corresponding to the M-beta fragment of the precursor protein, reported in scientific literature (PMID: 23389629). Calculated MW of 70.3 kDa band according to NP_008859.1
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PMEL
Long Name
Premelanosome Protein
Alternate Names
Gp100, ME20, ME20-M, ME20M, PMEL17, SILV
Gene Symbol
PMEL
UniProt
Additional PMEL Products
Product Documents for PMEL17/SILV Antibody
Product Specific Notices for PMEL17/SILV Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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